Arylformamidase Function: Difference between revisions

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From the DALI output, 2c7b was found to be the most similar structure to 2pbl having a Z-score of 23.4, 16% sequence identity and a root mean square deviation of 3.0. Conservation of the catalytic triad of 2cb7 as identified by ... was analysed for 2pbl (see figure 1).
The putative annotation which was initially provided ''on the basis of...'' prompted a literature search using the term 'arylformamidase'. Pabarcus et al. (2007) have analysed the function of an arylformamidase present in the liver of ''Mus musculus''. Arylformamidase has...
 
Ironically, from the BLAST results, the most similar sequence to 2pbl for which there was functional information available was the arylformamidase characterised by Pabarcus et al. 2007. Using site-directed mutagenesis, a catalytic triad was identified - S162, D247 and H279. To elucidate any functional similarity
 
[[Image:arylformamidase_alignment.png|thumb|none|framed|'''Figure 3:''' ''Conservation of the catalytic triad between Arylformamidase and 2pbl.'']]
 
[[Image:Arylformadisae_reaction.gif|none|framed|'''Figure 4:''' ''The reaction catalysed by Arylformamidase.'']]


  [[Image:2c7b_alignment.png|none|framed|'''Figure 1:''' ''Conservation of the catalytic triad between 2cb7 and 2pbl.'']]
  [[Image:2c7b_alignment.png|none|framed|'''Figure 1:''' ''Conservation of the catalytic triad between 2cb7 and 2pbl.'']]
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  [[Image:Carboxylesterase_reaction.gif|none|framed|'''Figure 2:''' ''The fundamental reaction catalysed by carboxylesterases.'']]
  [[Image:Carboxylesterase_reaction.gif|none|framed|'''Figure 2:''' ''The fundamental reaction catalysed by carboxylesterases.'']]
== Evidence from Similar Sequences ==
[[Image:arylformamidase_alignment.png|thumb|none|framed|'''Figure 3:''' ''Conservation of the catalytic triad between Arylformamidase and 2pbl.'']]
[[Image:Arylformadisae_reaction.gif|none|framed|'''Figure 4:''' ''The reaction catalysed by Arylformamidase.'']]


[[Arylformamidase | Return to the main page...]]
[[Arylformamidase | Return to the main page...]]

Revision as of 08:33, 31 May 2008

The putative annotation which was initially provided on the basis of... prompted a literature search using the term 'arylformamidase'. Pabarcus et al. (2007) have analysed the function of an arylformamidase present in the liver of Mus musculus. Arylformamidase has...

Ironically, from the BLAST results, the most similar sequence to 2pbl for which there was functional information available was the arylformamidase characterised by Pabarcus et al. 2007. Using site-directed mutagenesis, a catalytic triad was identified - S162, D247 and H279. To elucidate any functional similarity

Figure 3: Conservation of the catalytic triad between Arylformamidase and 2pbl.
Figure 4: The reaction catalysed by Arylformamidase.
Figure 1: Conservation of the catalytic triad between 2cb7 and 2pbl.

A review of the DALI output revealed many structures similar to 2pbl with little sequence identity. The most similar of these, 2cb7, is a thermophilic and thermostable carboxylesterase. The catalytic triad responsible for its activity has been identified, and in determining whether 2pbl shared similar functionality, conservation of the catalytic triad between the two proteins was assessed (see figure 1).

Figure 2: The fundamental reaction catalysed by carboxylesterases.

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