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| == STRUCTURAL ANALYSIS ==
| | Methods employed to obtain the analytical data was devided into three categories |
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| '''SBP 1 Amino Acid FASTA FORMAT Sequence'''
| | [[Methods for evolutionary analysis]] |
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| >2ECE:A|PDBID|CHAIN|SEQUENCE
| | [[Methods for structural analysis]] |
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| MAIVPFKRDPTFYPSPKMAMKAPPEDLAYVACLYTGTGINRADFIAVVDVNPKSETYSKIVHKVELPYINDELHHFGWNA
| | [[Methods for functional analysis]] |
| CSSALCPNGKPNIERRFLIVPGLRSSRIYIIDTKPNPREPKIIKVIEPEEVKKVSGYSRLHTVHCGPDAIYISALGNEEG
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| EGPGGILMLDHYSFEPLGKWEIDRGDQYLAYDFWWNLPNEVLVSSEWAVPNTIEDGLKLEHLKDRYGNRIHFWDLRKRKR
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| IHSLTLGEENRMALELRPLHDPTKLMGFINMVVSLKDLSSSIWLWFYEDGKWNAEKVIEIPAEPLEGNLPEILKPFKAVP
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| PLVTDIDISLDDKFLYLSLWGIGEVRQYDISNPFKPVLTGKVKLGGIFHRADHPAGHKLTGAPQMLEISRDGRRVYVTNS
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| LYSTWDNQFYPEGLKGWMVKLNANPSGGLEIDKEFFVDFGEARSHQVRLSGGDASSDSYCYP
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| | | Back to [[Selenium binding protein]] |
| [[Image:PBB_GE_SELENBP1_214433_s_at_fs.png|frame|border|center|Gene expression of the SELENBP1 gene based on gene atlas of the mouse and human protein -encoding transcriptomes-proc. Natl. Ac. Sci. 101 (16) 6062-7]]
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| [[Image:2ece_bio_r_500structure.jpg|frame|border|left|'''Figure 1''' X-ray structure of hypothetical selenium-binding protein from ''Sulfolobus tokodaii'', ST0059 ( http://www.proteopedia.org/wiki/index.php/2ece ) and the JenaLib Jmol viewer showing SBP 1 secondary structure spinning [http://www.imb-jena.de/cgi-bin/3d_mapping.pl?CODE=2ece&MODE=asymmetric]]
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| [[Image:SABLE.gif|frame|border|left|'''Figure 2''' SBP amino acid structure prediction derived from the SABLE server prediction- '''Query name''': SBP 1 ( 2ECE )
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| Structure prediction by SABLE
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| '''Data source''': Derived from the SABLE server prediction
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| ]]
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| '''WARNING!''' Given sequence appeared to be a soluble protein, no TM domains found!
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| Output format is the following:
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| '''1st line''' -> residue numeration
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| '''2nd line''' -> query amino acid sequence
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| '''3rd line''' -> trans-membrane domain prediction (T-TM region, '''N'''-soluble part)
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| MAIVPFKRDPTFYPSPKMAMKAPPEDLAYVACLYTGTGINRADFIAVVDVNPKSETYSKI
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| NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
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| VHKVELPYINDELHHFGWNACSSALCPNGKPNIERRFLIVPGLRSSRIYIIDTKPNPREP
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| NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
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| KIIKVIEPEEVKKVSGYSRLHTVHCGPDAIYISALGNEEGEGPGGILMLDHYSFEPLGKW
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| NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
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| EIDRGDQYLAYDFWWNLPNEVLVSSEWAVPNTIEDGLKLEHLKDRYGNRIHFWDLRKRKR
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| NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
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| IHSLTLGEENRMALELRPLHDPTKLMGFINMVVSLKDLSSSIWLWFYEDGKWNAEKVIEI
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| NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
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| PAEPLEGNLPEILKPFKAVPPLVTDIDISLDDKFLYLSLWGIGEVRQYDISNPFKPVLTG
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| NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
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| KVKLGGIFHRADHPAGHKLTGAPQMLEISRDGRRVYVTNSLYSTWDNQFYPEGLKGWMVK
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| NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
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| LNANPSGGLEIDKEFFVDFGEARSHQVRLSGGDASSDSYCYP
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| NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
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| '''Figure 3''' SABLE server results.
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| [[Image:movie0001.png]] | |
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| '''Figure 4''' Shows structural helices(red) and beta sheets (yellow) of SBP designed from pymol
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| [[Image:secondary structure.gif]]
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| '''Figure 5'''
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| [[Image:pdb_cartoon_2ece.png]]
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| '''Figure 6'''
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| STRUCTURAL COMPARISONS
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| [[Image:surface of 2ece.png]]
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| Surface Structure of SBP: Note the few clefts compared to the DNA isomerase shown below.
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| [[Image:1YUA SURFACE STRUCTURE.JPG]]]]]
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| [http://www.ncbi.nlm.nih.gov/entrez/query/static/gifs/entrez_struc.gif]
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| [[Image:Rat fatty acid binding protein 2IFB.jpg]]
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| Rat fatty acid binding protein 2IFB, a was found to have 92.5% homology to 14 KDa Selenium binding protein purified from rat liver using column chromatography and SDS-Gel techniques.([[http://compbio.chemistry.uq.edu.au/mediawiki/index.php/References_2ece ref 3]])
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| Explore SBP features and structural summary here [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?uid=61601].The domains of SBP are shown here [http://www.ncbi.nlm.nih.gov/sites/entrez?db=domains&cmd=search&term=2ece]
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| Notice how the domains are similar to the putative Isomerase domains of E.coli below.
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| '''1RI6 DOMAINS'''
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| [[Image:1RI6 A 0.png]]
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| [[Image:1RI6 A 1.png]]
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| [[Image:1RI6 A 2.png]]
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| [[Image:1RI6 A 3.png]]
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| '''2ECE DOMAINS'''
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| [[Image:2ece A 3.png]]
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| [[Image:2ece A 2.png]]
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| [[Image:2ece A 1.png]]
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| [[Image:2ece A 0.png]]
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| [[Image:Ligand of bovine.png]]
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| (Complex Of Bovine Odorant Binding Protein (Obp) With A Selenium Containing Odorant)"Image:Ligand of bovine.png" [[http://compbio.chemistry.uq.edu.au/mediawiki/upload/2/23/Ligand_of_bovine.png]]
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| == SEQUENCE ANALYSIS ==
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| Selenium binding protein 1 (SELENBP1)
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| SELECTED PROTEIN SIMILARITIES
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| Comparison of sequences in UniGene with selected protein reference sequences. The alignments can suggest function of a gene.
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| [http://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Hs&CID=632460]
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