Arylformamidase Function Slide 2: Difference between revisions

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== Evidence from Similar Sequences ==
== Evidence from Similar Sequences ==


The putative annotation which was initially provided ''on the basis of...'' prompted a literature search using the term 'arylformamidase'. Pabarcus et al. (2007) have analysed the function of an arylformamidase present in the liver of ''Mus musculus''.  
Screening of BLAST result (see Sebastien) for associated literature - resulted in the same paper as the literature search.
Ironically, from the BLAST results, the most similar sequence to 2pbl for which there was functional information available was the arylformamidase characterised by Pabarcus et al. 2007. Using site-directed mutagenesis, a catalytic triad was identified - S162, D247 and H279. To elucidate any functional similarity between arylformamidase and 2pbl, conservation of the catalytic triad was assessed through a clustalW alignment (see figure ...). Both S162 and H279 were found to be conserved in relatively conserved regions of the alignment whereas D247 had undergone a semi-conserved substitution. These residues correlated to... The residues were located on the tertiary structure of 2pbl and determined to be sufficiently proximal to one another to permit catalysis (see figure...).  
 
Catalytic triad identified in paper. To assess functional similarity, conservation of the catalytic triad was analysed.


[[Image:arylformamidase_alignment.png|centre|framed|'''Conservation of the catalytic triad between Arylformamidase and 2pbl.''']]
[[Image:arylformamidase_alignment.png|centre|framed|'''Conservation of the catalytic triad between Arylformamidase and 2pbl.''']]


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Revision as of 11:52, 8 June 2008

Evidence from Similar Sequences

Screening of BLAST result (see Sebastien) for associated literature - resulted in the same paper as the literature search.

Catalytic triad identified in paper. To assess functional similarity, conservation of the catalytic triad was analysed.

Conservation of the catalytic triad between Arylformamidase and 2pbl.

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