Arylformamidase Function Slide 4

Evidence from Similar Structures

- Screening of DALI result (see Basma) for functional information.

- Most similar structure well-characterised:

Byun JS, Rhee JK, Kim ND, Yoon J, Kim DU, Koh E, Oh JW, Cho HS.
Crystal structure of hyperthermophilic esterase EstE1 and the relationship between its dimerization and thermostability properties.
BMC Struct Biol. 2007 Jul 12;7:47.

- A thermostable carboxylesterase from an uncultured archaeon - isolated in an environmental sample.

Evidence from Similar Structures

- Catalytic triad identified in paper - Ser162, Asp247, and His279.

- To assess functional similarity, conservation of the catalytic triad was analysed.

catalytic triad has been identified (how?). To elucidate any functional similarity between 2pbl and 2c7b, conservation of the catalytic triad was assessed (see figure ...). Note: the clustalW alignment was found to differ from the alignment provided as part of the DALI results. All three residues were found to be conserved, though H... and E... were found to match is less conserved regions. Thus, it is possible that such conservation is the result of a chance or poor alignment. This might be explained by the poor sequence similarity between 2c7b and 2pdb (16%).

Conservation of the catalytic triad between 2cb7 and 2pbl.

Similarity in mechanism?

The reaction catalysed by Arylformamidase.

The fundamental reaction catalysed by carboxylesterases.

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