Pyridoxal Phosphatase Results
Evolution
Structure
Crystal structure of the target protein (2cfsA), as obtained from the Protein Data Bank (PDB)
Based on the information provided in the website, Pyridoxal Phosphatase has the following characteristics:
- Pyridoxal Phosphatase is isolated from Homo Sapiens and is expressed in Escherichia Coli.
- Structure is similar to the Pyridoxal Phosphate Phospatase protein
- 1 (A) Chain
- Consists of Magnesium components
http://www.rcsb.org/pdb/explore/explore.do?structureId=2cfs
DALI
A total of 176 hits were generated, of which only the first 11 (as shown above) were predicted to be significant. The others were rejected on account that their respective nres scores (refer to the red, boxed section in the diagram below) were less than half of Pyridoxal Phosphatase's (nres: 296).
It was noted that none of the hits actually matched Pyridoxal Phosphatase. The closest hit was a Pyridoxal Phosphate Phosphatase (PDB ID 2oycA) which bore great similarity to the protein of interest. Below is a table comparing both Pyridoxal Phosphatase (2cfsA) and the Pyridoxal Phosphate Phosphatase (2oycA)
Using the PyMOL software, 2oycA was superimposed against 2cfsA, and both structures, as shown below, are structurally similar.
Function