3bsqA Introduction

Sulfatases are enzymes that hydrolyze sulfate ester bonds of substrates. Seventeen genes are inentified coding for different human sulfatases; These are categorized as EC 3.1.6. in enzyme classifications. They all participate in metabolic processes, most of the family members are shown to contain a highly conserved cystine residue and a bivalent metal binding site. Majority of sulfatases are located in lysosomes with an acidic pH optima; however human steroid sulfatase is found in endoplasmic reticulums (ER) with a neutral pH potima. most of the sulfatases including Arylsulfatase A (ASA), Arylsulfatase B (ASB) and N-acetylgalactosamine-6-sulfatase (G6S) are water soluble.

The sulfatase in our focus is arylsulfatase K (ASK), where the crystal structure is been solves but no functional characterization is done to the date. In this experiment sequence conservation, structural similarity, other proteins that ASK is known to interact with, and the level of conservation of some key residues that may be involved in its catalytic activity were looked at; to determine the possible function of the ASK [[1]].

Function of some other sulfatases were found searching literature. ASA is a lysosomal enzyme which hydrolyzes cerebroside sulphate. ASB is also a lysosomal enzyme which hydrolyzes the sulphate ester group from N-acetylgalactosamine 4-sulphate group of dermatine sulphate. Arylsulfatase C is a microsomal membrane-bound enzyme hydrolyses 3-beta-hydroxysteroid sulfates which is also known as steroid sulfatase[[2]]. The range of substrates catalysed by sulfatases is diverse, including cerebroside sulfate, dermatan sulfate, heparan sulfate, heparin and keratan sulfate, and steroid sulfates [[3]]

Introduction Sulfatases are enzymes that hydrolyze sulfate ester bonds. Seventeen unique genes encoding sulfatases have been identified in humans.. These are categorized as EC 3.1.6. in enzyme classifications. They all participate in metabolic processes. Most of the family members contain a highly conserved cystine (C) residue and a bivalent metal binding site. The majority of sulfatases are located in lysosomes with acidic pH optima. Most of the sulfatases, including Arylsulfatase A (ASA), Arylsulfatase B (ASB) and N-acetylgalactosamine-6-sulfatase (G6S), are water soluble.

Looking at several sulfatases with a defined function will be helpful in appreciating the diversity of this enzyme family. ASA is a lysosomal enzyme which hydrolyzes cerebroside sulphate. ASB is also a lysosomal enzyme which hydrolyzes the sulphate ester group from N-acetylgalactosamine 4-sulphate group of dermatine sulphate. Arylsulfatase C (ASC) is a microsomal membrane-bound enzyme that hydrolyses 3-β-hydroxysteroid sulfates and is hence also known as steroid sulfatase (STS)2. The range of substrates hydrolysed by sulfatases include cerebroside sulfate, dermatan sulfate, heparan sulfate, heparin and keratan sulfate, and steroid sulfates 3 Previous investigation has revealed that arylsulfatases D, E, F, G, H, J and K are localized in ER and golgi compartments of the cell ([[4]] Ghosh, D., 2007).

The sulfatase studied in this experiment is arylsulfatase K (ASK); in which the crystal structure has been obtained, {[5]], but the substrates and function of this enzyme are unknown. Sequence conservation, structural similarity, other proteins that ASK is known to interact with, and the level of conservation of some key residues that may be involved in its catalytic activity, were examined in this experiment to determine the possible function of the ASK 1.