3bsqA Methods & Materials

Multiple sequence alugnment was made using clustalX from the DVD. The crystal structure of all proteins involved; ASA, ASK, STS; were viewed using the protein data bank text file downloaded to the programme PyMol ( Downloaded version in 'colaborative learning center' (CLC). The structural alignment was done using the DALI server using PDB identifiers. Protein interaction of ASK were searched with the programme 'STRING' which is available online. The protein name or PDB identifier was not detected by string, therefore the Aminoacid sequence was used. Functions of selected proteins were searched using ProFunc, which is also available online. Two sequence alignment of ASK and STS was done with the programme SIM.The methode used was 'BLOSUM62' and gap penalty and gap extension penalty were specifies to be 5 and 2 respectively.