Fascin Discussion: Difference between revisions
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The multiple alignment of fascin 1 supports research conducted by Wulfkuhle (1993), concluding the fascin contains strong sequence similarities between human, sea urchin, and Drosophilia. Furthermore, Fascin 1 contains 4 conserved PKC phosphorylation sites across the Eukaryotes tested. However despite the findings of Mitchison (2004), Fascin 1 was found not to be closely related to Protozoa, and their use of actin in actin-dependant propulsion. | The multiple alignment of fascin 1 supports research conducted by Wulfkuhle (1993), concluding the fascin contains strong sequence similarities between human, sea urchin, and Drosophilia. Furthermore, Fascin 1 contains 4 conserved PKC phosphorylation sites across the Eukaryotes tested. However despite the findings of Mitchison (2004), Fascin 1 was found not to be closely related to Protozoa, and their use of actin in actin-dependant propulsion. | ||
Despite Mitchison (2004) findings, phylogenetic researched conducted did not support his findings that some Protozoa contain fascin homolog regions. Two trees were created to prove/disprove his conclusion, the first was created straight from a multiple alignment of 100 sequences, with multiple alignments from the same organism deleted. The second however was a selective tree, with PKC phosphorylation sites viewed and unconserved or gapped sequences cleared. | |||
The phylogenetic tree further illustrates the conservation of Facin 1 throughout the Eukaryotes. Although distant, the bootstrap values indicate that the Insecta and Animalia branches are closely related and supports Wulfkuhle (1993) conclusion that Drosophilia, Homo sapien and Strongylocentrotus purpuratus contain a fascin homolog. Fascin binds beta-catenin, a sub-unit of the cadherin protein complex and is important in Wnt signalling pathway and therefore is highly conserved throughout Eukaryotes | No Bacteria or Archea were found in the broad tree, therefore it can be concluded that Fascin 1 is a Eukaryote specific actin-binding protein. Across the Eukaryotes, Fascin 1 was highly conserved, particularly at the PKC phosphorylation sites. This shows that the sites contain important regions for functional and structural integrity. | ||
The phylogenetic tree further illustrates the conservation of Facin 1 throughout the Eukaryotes. Although distant, the bootstrap values indicate that the Insecta and Animalia branches are closely related and supports Wulfkuhle (1993) conclusion that Drosophilia, Homo sapien and Strongylocentrotus purpuratus contain a fascin homolog. Fascin binds beta-catenin, a sub-unit of the cadherin protein complex and is important in Wnt signalling pathway and therefore is highly conserved throughout Eukaryotes. | |||
Revision as of 23:01, 14 June 2009
Discussion
The multiple alignment of fascin 1 supports research conducted by Wulfkuhle (1993), concluding the fascin contains strong sequence similarities between human, sea urchin, and Drosophilia. Furthermore, Fascin 1 contains 4 conserved PKC phosphorylation sites across the Eukaryotes tested. However despite the findings of Mitchison (2004), Fascin 1 was found not to be closely related to Protozoa, and their use of actin in actin-dependant propulsion.
Despite Mitchison (2004) findings, phylogenetic researched conducted did not support his findings that some Protozoa contain fascin homolog regions. Two trees were created to prove/disprove his conclusion, the first was created straight from a multiple alignment of 100 sequences, with multiple alignments from the same organism deleted. The second however was a selective tree, with PKC phosphorylation sites viewed and unconserved or gapped sequences cleared.
No Bacteria or Archea were found in the broad tree, therefore it can be concluded that Fascin 1 is a Eukaryote specific actin-binding protein. Across the Eukaryotes, Fascin 1 was highly conserved, particularly at the PKC phosphorylation sites. This shows that the sites contain important regions for functional and structural integrity.
The phylogenetic tree further illustrates the conservation of Facin 1 throughout the Eukaryotes. Although distant, the bootstrap values indicate that the Insecta and Animalia branches are closely related and supports Wulfkuhle (1993) conclusion that Drosophilia, Homo sapien and Strongylocentrotus purpuratus contain a fascin homolog. Fascin binds beta-catenin, a sub-unit of the cadherin protein complex and is important in Wnt signalling pathway and therefore is highly conserved throughout Eukaryotes.
