Pyridoxal Phosphatase function

Vitamin B6, Pyridoxal Phosphatase,

General Annotation

Fig 17. General annotation of Pyridoxal Phosphatase. Picture courtesy of UniProt

Known Functions

Amino Acid Metabolism

2cfsA is involved in almost all amino acid metabolism. Some of these amino acid metabolism include transamination, transsulfuration, selenoamino acid metabolism and conversion of typtophan to niacin.

Gluconeogenesis

2cfsA can catalyse transamination reactions that are essential for providing amino acids as a substrate for gluconeogenesis. 2cfsA is also required as a coenzyme for glycogen phosphorylase, which in turn catalyse glycogenolysis.

Neurotransmitter Synthesis

Pyridoxal phosphate-dependent enzymes catalyses the biosynthesis of serotonin, epinephrine, norepinephrine and gamma-aminobutyric acid(The four important neurotranmsitters).

Histamine Synthesis

2cfsA is involved in the metabolism of histamine.

Hemoglobin Synthesis and Function

Pyridoxal phosphate aids in the synthesis of heme and can also bind to two sites on hemoglobin to enhance the oxygen binding of hemoglobin.

Gene Expression

Pyridoxal phosphate has been implicated in increasing or decreasing the expression of certain genes. Increased intracellular levels of 2cfsA will lead to a decrease in the transcription of glucocorticoid hormones. Also, vitamin B6 deficiency will lead to the increased expression of albumin mRNA. Also, pyridoxal phosphate will influence gene expression of glycoprotein IIb by interacting with various transcription factors. The result is inhibition of platelet aggregation.

Sequence homology

Fig 18. Results from FASTA showing five highly related enzymes to 2cfsA

Structural Similarities

Fig 19. Search results using Interpro for similar protein structure based on motifs.

Location of Gene on Genome

Fig 20. Gene location results from Profunc showing two neighbouring genes of 2cfsA with very different sequence but similar function.

Functional Expression in Tissue

Fig 21. Tissue specific expression of the human PLP phosphatase mRNA. The human multiple tissue expression array was hybridized with human PLP phosphatase-specific DNA probe. Tissue sources for the RNA are indicated below the dot.

Protein-Protein Interactions

Fig 22. Diagram on protein-protein interaction of 2cfsA.

Functional Prediction:

Cofactor of HSPD1 and play a role in protein folding.

Coenzyme for asparagine synthetase by interacting with TS11.

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