Structure: Difference between revisions

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[http://pubs.acs.org/cgi-bin/article.cgi/achre4/2004/37/i10/pdf/ar040002e.pdf Unravelling the structure and mechanism of Acetyl-Coenzyme A Synthase]
== Materials & Methods==
1) Protein structural search on PyMOL, NCBI Entrez, InterPro and PDB databases
* PDB code: 3b5q
2) Comparing protein search with DALI server to identify similiar structures


[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T36-44C8PCW-5M&_user=331728&_coverDate=08%2F05%2F1985&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000016898&_version=1&_urlVersion=0&_userid=331728&md5=1346047af0e050435336343459012fc7 Limited Proteolysis of Pig Liver CoA synthase: Evidence for Subunit Identity]
 
 
== Results ==
'''PDB'''
 
[[Image:3b5q image PDB.png|thumb|'''Figure 1.0''' Structure of 3b5q]]
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
== Discussion ==
 
 
'''Sequence'''
 
>gi|160286517|pdb|3B5Q|A Chain A, Crystal Structure Of A Putative Sulfatase (Np_810509.1) From Bacteroides Thetaiotaomicron Vpi-5482 At 2.40 A Resolution
GXGLALCGAAAQAQEKPNFLIIQCDHLTQRVVGAYGQTQGCTLPIDEVASRGVIFSNAYVGCPLSQPSRA
ALWSGXXPHQTNVRSNSSEPVNTRLPENVPTLGSLFSESGYEAVHFGKTHDXGSLRGFKHKEPVAKPFTD
PEFPVNNDSFLDVGTCEDAVAYLSNPPKEPFICIADFQNPHNICGFIGENAGVHTDRPISGPLPELPDNF
DVEDWSNIPTPVQYICCSHRRXTQAAHWNEENYRHYIAAFQHYTKXVSKQVDSVLKALYSTPAGRNTIVV
IXADHGDGXASHRXVTKHISFYDEXTNVPFIFAGPGIKQQKKPVDHLLTQPTLDLLPTLCDLAGIAVPAE
KAGISLAPTLRGEKQKKSHPYVVSEWHSEYEYVTTPGRXVRGPRYKYTHYLEGNGEELYDXKKDPGERKN
LAKDPKYSKILAEHRALLDDYITRSKDDYRSLKVDADPRCRNHTPGYPSHEGPGAREILKRK
 
== Links ==
 
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&val=160286517 Arylsulfatase sequence]
 
[http://www.ncbi.nlm.nih.gov/pubmed/17558559?ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Human sulfatases: a structural perspective to catalysis]
 
[http://www.ncbi.nlm.nih.gov/pubmed/10212197?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DiscoveryPanel.Pubmed_Discovery_RA&linkpos=4&log$=relatedarticles&logdbfrom=pubmed  Amino acid residues forming the active site of arylsulfatase A. Role in catalytic activity and substrate binding]


[http://www.rcsb.org/pdb/explore/explore.do?structureId=3b5q Protein structure in PDB]
[http://www.rcsb.org/pdb/explore/explore.do?structureId=3b5q Protein structure in PDB]




[[Image:2gfh asym r 500.jpg|thumb|2GFH]]
'''Crystal Structure'''
[[Image:protein showing zinc pymol.png|300px|thumb]]

Latest revision as of 01:25, 21 May 2008

Materials & Methods

1) Protein structural search on PyMOL, NCBI Entrez, InterPro and PDB databases

  • PDB code: 3b5q

2) Comparing protein search with DALI server to identify similiar structures


Results

PDB

Figure 1.0 Structure of 3b5q












Discussion

Sequence

>gi|160286517|pdb|3B5Q|A Chain A, Crystal Structure Of A Putative Sulfatase (Np_810509.1) From Bacteroides Thetaiotaomicron Vpi-5482 At 2.40 A Resolution GXGLALCGAAAQAQEKPNFLIIQCDHLTQRVVGAYGQTQGCTLPIDEVASRGVIFSNAYVGCPLSQPSRA ALWSGXXPHQTNVRSNSSEPVNTRLPENVPTLGSLFSESGYEAVHFGKTHDXGSLRGFKHKEPVAKPFTD PEFPVNNDSFLDVGTCEDAVAYLSNPPKEPFICIADFQNPHNICGFIGENAGVHTDRPISGPLPELPDNF DVEDWSNIPTPVQYICCSHRRXTQAAHWNEENYRHYIAAFQHYTKXVSKQVDSVLKALYSTPAGRNTIVV IXADHGDGXASHRXVTKHISFYDEXTNVPFIFAGPGIKQQKKPVDHLLTQPTLDLLPTLCDLAGIAVPAE KAGISLAPTLRGEKQKKSHPYVVSEWHSEYEYVTTPGRXVRGPRYKYTHYLEGNGEELYDXKKDPGERKN LAKDPKYSKILAEHRALLDDYITRSKDDYRSLKVDADPRCRNHTPGYPSHEGPGAREILKRK

Links

Arylsulfatase sequence

Human sulfatases: a structural perspective to catalysis

Amino acid residues forming the active site of arylsulfatase A. Role in catalytic activity and substrate binding

Protein structure in PDB


Crystal Structure

Protein showing zinc pymol.png