3bsqA Results

MSA highlited sevlral residues which are very highly conserves (figure 1).

The three dimentional structure was viewed using PyMol (figure 2) and these conserved residues were marked on the crystal structure. However they were not related to binding sited of Cl and Zn, but buried in the core of the protein within a pocket-like region (figure 3). All attempts to see the electrostatic nature of this pocket were unsuccessful, due to some technical probloems with PyMol.

Structural alignement of arylsulfatase K using DALI resulted several other sulfatases (figure 4).

 No:  Chain   Z    rmsd lali nres  %id   Description
  1:  3b5q-A 73.6  0.0  464   464  100   MOLECULE: PUTATIVE SULFATASE YIDJ;                                   
  2:  3b5q-B 70.2  0.3  464   467  100   MOLECULE: PUTATIVE SULFATASE YIDJ;                                   
  3:  2qzu-A 35.1  2.5  375   465   25   MOLECULE: PUTATIVE SULFATASE YIDJ;                                   
  4:  1fsu   28.7  2.8  344   474   22   MOLECULE: N-ACETYLGALACTOSAMINE-4-SULFATASE;                         
  5:  1n2l-A 28.4  3.0  343   483   25   MOLECULE: ARYLSULFATASE A;                                           
  6:  1n2k-A 28.3  3.2  344   482   25   MOLECULE: ARYLSULFATASE A;                                           
  7:  1e3c-P 28.2  3.2  344   481   26   MOLECULE: ARYLSULFATASE A;                                           
  8:  1e33-P 28.2  3.1  344   480   25   MOLECULE: ARYLSULFATASE A;                                           
  9:  1e2s-P 28.2  3.1  343   481   25   MOLECULE: ARYLSULFATASE A;                                           
 10:  1e1z-P 28.1  3.2  344   481   26   MOLECULE: ARYLSULFATASE A;                                           
 11:  1auk   27.9  3.1  343   481   25   MOLECULE: ARYLSULFATASE A;  

Figure 4: Structurally related proteins. (No 1 and 2 are two chains of arylsulfatase K).

Protein interactions of arylsulfatase K with other proteis were vieved usig the server STRING, which revealed four major hits, depending on XXX and XXX evidence.